Overview

Highlights

  • Automated de novo antibody sequencing
  • Quantification of modifications and variants
  • Q/CQA of antibody production
  • Comprehensive report generation

The newest release of PEAKS AB Software, version 2.0, is equipped with new tools to further improve sequencing accuracy. It also provides a more convenient and user-friendly workflow for antibody sequencing analysis with new customization capabilities.


Monoclonal antibodies have a wide range of applications in biochemical analyses, medical research, diagnostics and therapeutics. The protein sequence of an antibody (Ab) encodes critical information for its structure and function. When cDNA or the hybridoma cell line is not available, de novo protein sequencing of an antibody can help.

PEAKS AB Software is a platform for de novo antibody sequencing and the characterization of modifications and sequence variants by using liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS). PEAKS AB software also supports characterization of post-translational modifications (PTMs) and variants for research discovery and quality control purposes.

PEAKS AB software directly sequences the antibody protein using high resolution LC-MS/MS data sets from orthogonal enzyme digests. It performs de novo peptide sequencing first, and assembles the confident sequence tags into antibody sequences.

Screen Shot 2019-12-05 at 9.34.50 AM

  1. Antibody Sample Description
  2. Abbreviations
  3. Experimental Procedure
  4. Antibody Heavy Chain
    1. Amino Acid Sequence
    2. Peptide Mapping at 0.1% of FDR at Peptide-Spectrum Level
    3. Typical Peptides Selected for Variable Region
    4. Ile/Leu Differentiation
peaksab-reportgeneration
  1. Antibody Light Chain
    1. Amino Acid Sequence
    2. Peptide Mapping at 0.1% of FDR at Peptide-Spectrum Level
    3. Typical Peptides Selected for Variable Region
    4. Ile/Leu Differentiation