README
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This file includes raw data information and search parameters used for PEAKS demo project. 

OVERVIEW
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Project name: Orbi_DDA_LFQ_Individual
Create Date: June 2, 2020
PEAKS Version: Xplus, 20200415

RAW DATA
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Retrieved from: Bekker-Jensen, D.B., et.al. (2020).
https://doi.org/10.1074/mcp.TIR119.001906
PXD016662

Files used in project:
20190521_QE12_EVO2_AMV_LFQ-5ng_5min_15000_01
20190521_QE12_EVO2_AMV_LFQ-5ng_5min_15000_02
20190521_QE12_EVO2_AMV_LFQ-5ng_5min_15000_03
20190521_QE12_EVO2_AMV_LFQ-50ng_5min_15000_01
20190521_QE12_EVO2_AMV_LFQ-50ng_5min_15000_02
20190521_QE12_EVO2_AMV_LFQ-50ng_5min_15000_03
20190521_QE12_EVO2_AMV_LFQ-500ng_5min_15000_01
20190521_QE12_EVO2_AMV_LFQ-500ng_5min_15000_02
20190521_QE12_EVO2_AMV_LFQ-500ng_5min_15000_03

DATA REFINEMENT
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Selected:
Correct Precusor on Mass Only
Associate features with chimera scans
Filter features, charge between 2-8

DATABASE SEARCH PARAMETERS
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Database: Human UniProt Reviewed

Parent Error Tolerance: 10ppm
Fragment Error Tolerance: 0.02 ppm

Enzyme: Trypsin,
Digest Mode: Specific 
2 missed cleavages

Modications Selected:
Fixed:+57 Carbamidomethylation (C)
Variable: +42 Aceytlation (Protein N-Term), +15.99 Oxidation (M), +0.98 Deamidation (NQ)
Set maximum of 2 variable PTM/peptide