Oliviero, G., et al. Easy, fast and reproducible analysis of host cell protein (HCP) in monoclonal antibody preparations Application Note 21918
To perform host cell protein analysis using an automated, rapid digestion method based on the Thermo Scientific™ SMART Digest™ kit on a Thermo Scientific™ KingFisher™ Duo Prime purification system. Sample analysis is performed by LC-MS on the Thermo Scientific™ Vanquish™ UHPLC coupled to the Thermo Scientific™ Q Exactive™ Plus Hybrid Quadrupole-Orbitrap™ mass spectrometer. To show the flexibility of the automated platform that can be employed for both peptide mapping analysis and HCP characterization.