• Design and perform LC-MS/MS analysis (≥50μg of a purified protein)
  • Guarantee accuracy by multiple proofs
  • Guarantee two-week turn-around and service quality

Why is Ile/Leu differentiation needed?

How to Differentiate Ile/Leu?

1. Johnson, R. S., Martin, S., Biemann, K., Stults, J. T., & Watson, J. T. Novel fragmentation process of peptides by collision-induced decomposition in a tandem mass spectrometer: differentiation of leucine and isoleucine. Anal Chem. 1987 Nov 1;59(21):2621-5.
2. Xiao, Y., Vecchi, M. M., & Wen, D. Distinguishing between Leucine and Isoleucine by Integrated LC-MS Analysis using an Orbitrap Fusion Mass Spectrometer. Anal Chem. 2016 Nov 1;88(21):10757-10766.
3. Zhokhov, S. S., Kovalyov, S. V., Samgina, T. Y., & Lebedev, A. T. An EThcD-Based Method for Discrimination of Leucine and Isoleucine Residues in Tryptic Peptides. J Am Soc Mass Spectrom. 2017 Aug;28(8):1600-1611.