Antibody Sequencing Service

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PEAKS AB Service

Key Features:

  • Design and perform LC-MS/MS analysis (0.2 mg sample)
  • de novo protein sequencing
  • Works for all antibody isotypes and species
  • Quantify PTMs and sequence variants
  • Guarantee two-week turn-around and service quality

Book PEAKS AB - de novo Antibody Sequencing Service

Workflow of de novo Antibody Sequencing

de novo antibody protein sequencing service workflow

BSI has developed the proprietary de novo antibody sequencing technology, based on our PEAKS AB Software, to sequence antibodies. The Antibody Sequencing Service consists of full length heavy and light-chain antibody sequencing for all species, isotypes and allotypes. Numerous successful cases from our antibody protein sequencing service have confirmed 100% accuracy and 100% coverage. Satisfaction is guaranteed to meet our customers’ needs.

PEAKS AB- de novo antibody protein sequencing service full coverage

100% Sequence Coverage

Our Antibody Sequencing Service guarantees each amino acid is typically mapped with more than 20 peptides. The peptide mapping in the left is obtained using our PEAKS AB Software at 0.1% of FDR at peptide-spectrum level. Each bar under the sequence denotes a peptide identified from the MS/MS data and the color represents a specified enzyme used for sample digestion.

100% Sequence Accuracy

The Antibody Protein Sequencing Service carries out quality control at the amino acid level based on the de novo sequencing result. Direct fragment ion evidences from MS/MS data are required for each amino acid in the assembled protein sequences.

PEAKS AB - de novo Antibody Sequencing View

Antibody Sequencing Service - Modification Analysis

Modification Analysis

The Antibody Protein Sequencing Service provides qualitative and quantitative analysis for post-translational modifications (PTMs) and sequence variants. The screenshot illustrates a PTM analysis result using our PEAKS AB Software.

Antibody Sequencing Service - Leu/Ile Differentiation

Leucine & Isoleucine Differentiation

The Antibody Sequencing Service differentiates leucine and isoleucine residues based on the enzyme cleavage preference and homology database statistics.

Antibody Sequencing Service - Leucine & Isoleucine Differentiation