A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis

Clement et al. have published their method to identify differences in endosomal antigen processing by dendritic cells including wet lab, LC-MS/MS & data analysis. Clear and concise details in this publication, including step-by-step LC-MS/MS and bioinformatic instruction, make this paper an excellent guide for researchers interested in the identification and quantification of antigenic proteins and peptide epitopes.

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