Extending the Mass Spectrometry-Detectable Landscape of MHC Peptides by Use of Restricted Access Material

For MS-based immunopeptidomics, major histocompatibility complex (MHC) peptides are typically isolated by immunoaffinity purification using specific anti-MHC antibodies. In this work, restricted access material (RAM)-SPE (solid-phase extraction) to increase the number of identified MHC peptides compared to C18-SPE.

Covalent Immune Proximity-Induction Strategy Using SuFEx-Engineered Bifunctional Viral Peptides

Covalent antibody recruiting molecules are synthetic bifunctional molecules capable of inducing antibodies to recognise and eliminate cancer cells by the innate immune machinery. Learn more about this proof-of-concept research by McCann et al, and how a “covalent immune proximity-induction” strategy may enable the efficient recruitment of immune receptors via the incorporation of available modest affinity peptide ligands.

A Comprehensive Study of Gradient Conditions for Deep Proteome Discovery in a Complex Protein Matrix

Bottom–up mass-spectrometry-based proteomics is a well-developed technology but the gradient type and time can have influence on the results and must be well studied. Learn how Wei et al. investigated various gradient types, and gradient lengths with different sample loading amounts and loading conditions to optimise the LC step.

Bovine Bone Gelatin-Derived Peptides: Food Processing Characteristics and Evaluation of Antihypertensive and Antihyperlipidemic Activities

Hypertension affects over a billion people around the world and overactivation of renin−angiotensin−aldosterone system (RAS) is one of the main pathologies. Here, this study aims to investigate the effects and mechanisms of bovine bone gelatin-derived peptides (BGPs) on hypertension and hypertension complications.

Understanding the constitutive presentation of MHC class I immunopeptidomes in primary tissues

CD8+ T cells can eradicate abnormal cells through the recognition of small peptides presented by major histocompatibility complex (MHC) class I molecules. In this study, a system-level and cross-species analysis of the MHC-I immunopeptidome was conducted to understand how the MHC-I immunopeptidome is being shaped in different primary tissues in vivo.