CD8+ T cells can eradicate abnormal cells through the recognition of small peptides presented by major histocompatibility complex (MHC) class I molecules. In this study, a system-level and cross-species analysis of the MHC-I immunopeptidome was conducted to understand how the MHC-I immunopeptidome is being shaped in different primary tissues in vivo.
Umami is the fifth taste found in substances that mainly include amino acids and nucleotides. Here, the combination of peptidomics and virtual screening is employed for rapid screening of samples to identify novel umami peptides in clam species.
E-cigarettes have gained popularity as a safer alternative to cigarettes; but research has shown the harmful effects of e-cigarettes on a range of cellular processes. In this study, the researchers investigated the effects of e-cigarette liquid with or without nicotine on V79 lung fibroblast cells.
The liver is the main venue for β-oxidation of fatty acids, and it maintains the balance of fat metabolism. Many studies showed that betaine regulates fat metabolism. In this work, the researchers investigated the protein metabolic profile in the liver of mice on an HF diet supplemented with 1% betaine by label free quantification analysis via LC–MS/MS.
The Pacific oyster is a major seafood around the world and it is important to understand the effect of heating on proteins of this aquatic species. Learn how LC-MS/MS was used and how studies on food processing can improve the safety of food and reduce health-related costs.
Lactylation is a post translation modification (PTM) derived from lactate and here, researchers have shown that lactylation is a widespread PTM that is not restricted to histones and have developed a sensitive and validated method for lactylation identification by liquid chromatography–tandem mass spectrometry (LC–MS/MS).
Glioblastoma (GBM) is the most common and malignant brain tumour. Learn how LC-MS/MS is proving to be a beneficial method to understanding of brain extracellular matrix changes in GBM pathogenesis and discovery of novel makers and therapeutic targets.
Alterations in the matrisome, including collagens, glycoproteins, and proteoglycans, have been shown to be associated with neurodegenerative disorders. Here researchers investigated the alterations in the expression and modification of the matrisome.
Though there appears to be both genetic and environmental factors that influence the development of Myopia or nearsightedness, the precise mechanism remains unknown. In this study the research group aimed to analyze the exosomal protein profile of the aqueous humor (AH) of myopia patients to provide insight for the diagnosis, clinical treatment and prognosis of myopia.
Immunotherapy, like Immune checkpoint blockade (ICB), has been widely used with chemotherapy to improve cancer treatment. Since cannabis is used to treat nausea caused by chemotherapy, Xiong et al. studied if THC interferes with ICB treatment through the cannabinoid receptor 2 (CNR2) pathway.