For MS-based immunopeptidomics, major histocompatibility complex (MHC) peptides are typically isolated by immunoaffinity purification using specific anti-MHC antibodies. In this work, restricted access material (RAM)-SPE (solid-phase extraction) to increase the number of identified MHC peptides compared to C18-SPE.
Listeria is a foodborne pathogen that can cause listeriosis, which can be dangerous specially among vulnerable individuals including immunocompromised individuals. Learn how this study extended the antigen knowledge on Listeria, by applying an immunopeptidomics study on two infected human epithelial cell lines.
Covalent antibody recruiting molecules are synthetic bifunctional molecules capable of inducing antibodies to recognise and eliminate cancer cells by the innate immune machinery. Learn more about this proof-of-concept research by McCann et al, and how a “covalent immune proximity-induction” strategy may enable the efficient recruitment of immune receptors via the incorporation of available modest affinity peptide ligands.
Bottom–up mass-spectrometry-based proteomics is a well-developed technology but the gradient type and time can have influence on the results and must be well studied. Learn how Wei et al. investigated various gradient types, and gradient lengths with different sample loading amounts and loading conditions to optimise the LC step.
Hypertension affects over a billion people around the world and overactivation of renin−angiotensin−aldosterone system (RAS) is one of the main pathologies. Here, this study aims to investigate the effects and mechanisms of bovine bone gelatin-derived peptides (BGPs) on hypertension and hypertension complications.
CD8+ T cells can eradicate abnormal cells through the recognition of small peptides presented by major histocompatibility complex (MHC) class I molecules. In this study, a system-level and cross-species analysis of the MHC-I immunopeptidome was conducted to understand how the MHC-I immunopeptidome is being shaped in different primary tissues in vivo.
Umami is the fifth taste found in substances that mainly include amino acids and nucleotides. Here, the combination of peptidomics and virtual screening is employed for rapid screening of samples to identify novel umami peptides in clam species.
E-cigarettes have gained popularity as a safer alternative to cigarettes; but research has shown the harmful effects of e-cigarettes on a range of cellular processes. In this study, the researchers investigated the effects of e-cigarette liquid with or without nicotine on V79 lung fibroblast cells.