The glucose-dependent insulinotropic polypeptide is secreted from enteroendocrine cells and can enhance the release of insulin from pancreatic cells. The identification of peptides in human plasma are a challenging task and is barely studied by the scientists. Learn about this novel technology that can improve the plasma extraction yield, leading to the identification of trace peptides with low molecular weight using a single drop of plasma sample.
Extending the Mass Spectrometry-Detectable Landscape of MHC Peptides by Use of Restricted Access Material
For MS-based immunopeptidomics, major histocompatibility complex (MHC) peptides are typically isolated by immunoaffinity purification using specific anti-MHC antibodies. In this work, restricted access material (RAM)-SPE (solid-phase extraction) to increase the number of identified MHC peptides compared to C18-SPE.
Immunopeptidomics-based design of mRNA vaccine formulations against Listeria monocytogenes
Listeria is a foodborne pathogen that can cause listeriosis, which can be dangerous specially among vulnerable individuals including immunocompromised individuals. Learn how this study extended the antigen knowledge on Listeria, by applying an immunopeptidomics study on two infected human epithelial cell lines.
Covalent Immune Proximity-Induction Strategy Using SuFEx-Engineered Bifunctional Viral Peptides
Covalent antibody recruiting molecules are synthetic bifunctional molecules capable of inducing antibodies to recognise and eliminate cancer cells by the innate immune machinery. Learn more about this proof-of-concept research by McCann et al, and how a “covalent immune proximity-induction” strategy may enable the efficient recruitment of immune receptors via the incorporation of available modest affinity peptide ligands.
A Comprehensive Study of Gradient Conditions for Deep Proteome Discovery in a Complex Protein Matrix
Bottom–up mass-spectrometry-based proteomics is a well-developed technology but the gradient type and time can have influence on the results and must be well studied. Learn how Wei et al. investigated various gradient types, and gradient lengths with different sample loading amounts and loading conditions to optimise the LC step.
Regulation of Schwann Cell and DRG Neurite Behaviors within Decellularized Peripheral Nerve Matrix
Learn more about this study on how decellularized nerve hydrogels (dNHs) can be used for peripheral nerve injury and examining how dNHs can promote neurite remylination and inhibit synaptogenesis.
Bovine Bone Gelatin-Derived Peptides: Food Processing Characteristics and Evaluation of Antihypertensive and Antihyperlipidemic Activities
Hypertension affects over a billion people around the world and overactivation of renin−angiotensin−aldosterone system (RAS) is one of the main pathologies. Here, this study aims to investigate the effects and mechanisms of bovine bone gelatin-derived peptides (BGPs) on hypertension and hypertension complications.
Overexpressing CrePAPS Polyadenylate Activity Enhances Protein Translation and Accumulation in Chlamydomonas reinhardtii
Learn how overexpression of Canonical C. reihardtii poly (A) polymerase (crePAPSs) genes can possibly be harnessed to exploit green algae for recombinant protein production on an industrial scale.
Understanding the constitutive presentation of MHC class I immunopeptidomes in primary tissues
CD8+ T cells can eradicate abnormal cells through the recognition of small peptides presented by major histocompatibility complex (MHC) class I molecules. In this study, a system-level and cross-species analysis of the MHC-I immunopeptidome was conducted to understand how the MHC-I immunopeptidome is being shaped in different primary tissues in vivo.
Umami peptides screened based on peptidomics and virtual screening from Ruditapes philippinarum and Mactra veneriformis clams
Umami is the fifth taste found in substances that mainly include amino acids and nucleotides. Here, the combination of peptidomics and virtual screening is employed for rapid screening of samples to identify novel umami peptides in clam species.