Most non-canonical proteins uniquely populate the proteome or immunopeptidome

Translation events that were previously thought to result in non-biologically relevant proteins are now being reconsidered. Increasing evidence shows that some non-canonical proteins may play an important role in immunity and disease. This paper examines the unique features of the non-canonical translatome and how they may influence tumor immunosurveillance.

Proteogenomic Analysis Unveils the HLA Class I-Presented Immunopeptidome in Melanoma and EGFR-Mutant Lung Adenocarcinoma

One of the hurdles in the development of immunopeptidomic tools is the heterogeneity of the tumour-associated signals resulting in challenges with traditional database search strategies for MS data. Here Qi et al. from NIH are working to remove this hurdle by combining de novo peptide antigen sequencing and custom database strategies.