There is a growing interest for the potential of short open reading frames (sORFs), and the biological relevance of the translation products. Recently, Peeters et al., describe a workflow for investigating neuropeptides using timsTOF Pro with PASEF, database searches with a custom proteogenomic database, and machine learning tools to identify sORF-encoded peptides.
Methionine oxidation is a reversible Post-translational modifications (PTM) that can occur as part of cellular redox-regulation, which may influence protein structure, stability, and function. In a recent paper by Baumans et al., researchers look at different reversed-phase (RP) variables, to determine if any of these factors influenced the amount of processing-related methionine oxidation.
Fermented dairy products have traditionally been used in many cultures for their health promoting effects. In a recent study by Nielsen and others, researchers compare chemically acidified, live and heat-inactivated fermented dairy yoghurt and analyzed the peptides in these products by LC-MS/MS.