Ion Mobility Coupled to a Time-of-Flight Mass Analyzer Combined With Fragment Intensity Predictions Improves Identification of Classical Bioactive Peptides and Small Open Reading Frame-Encoded Peptides

There is a growing interest for the potential of short open reading frames (sORFs), and the biological relevance of the translation products. Recently, Peeters et al., describe a workflow for investigating neuropeptides using timsTOF Pro with PASEF, database searches with a custom proteogenomic database, and machine learning tools to identify sORF-encoded peptides.

Liquid chromatography setup-dependent artefactual methionine oxidation of peptides: The importance of an adapted quality control process

Methionine oxidation is a reversible Post-translational modifications (PTM) that can occur as part of cellular redox-regulation, which may influence protein structure, stability, and function. In a recent paper by Baumans et al., researchers look at different reversed-phase (RP) variables, to determine if any of these factors influenced the amount of processing-related methionine oxidation.

Chemically acidified, live and heat-inactivated fermented dairy yoghurt show distinct bioactive peptides, free amino acids and small compounds profiles

Fermented dairy products have traditionally been used in many cultures for their health promoting effects. In a recent study by Nielsen and others, researchers compare chemically acidified, live and heat-inactivated fermented dairy yoghurt and analyzed the peptides in these products by LC-MS/MS.