Validation of Trifluoromethylphenyl Diazirine Cholesterol Analogues As Cholesterol Mimetics and Photolabeling Reagents

In a recent article by Krishnan et al., researchers use middle-down proteomics to assess different cholesterol mimetics as photolabeling reagents to evaluate protein-protein interactions in situ. This study demonstrates the use of PEAKS Studio for analyzing middle-down data and identify where the various mimetics bound to their model protein Gloeobacter ligand-gated ion channel.

Disturbed mitochondrial acetylation in accordance with the availability of acetyl groups in hepatocellular carcinoma

Acetylation is an important post-translational modification (PTM) and can affect protein activity with a wide range of effects. In a study by Hu and colleagues, demonstrates the utility of global analysis tools, like proteomics for studying complex disease states, to explore the link between acetylation and metabolism in the tumor microenvironment.

Influence of surface termination of ultrananocrystalline diamond films coated on titanium on response of human osteoblast cells: A proteome study

Cell-based assays can be a useful tool for predicting the biocompatibility of different materials used for medical implants. In a recent study Merker et al. assessed the proteome of osteoblasts involved in transplant integration to further understand how these integral processes can be optimised to increase the chances of successful integration.