Bond, J.J., et al. Protein profiles of enzymatically isolated rumen epithelium in sheep fed a fibrous diet. Journal of Animal Science and Biotechnology. 10(5). 25/1/2019.
Our research has developed a procedure to isolate rumen epithelium proteins from the underlying tissue layers so that they may be profiled using proteomic technologies. The approach improves the number of proteins that can be profiled that are specific to the epithelium of the rumen wall. It provides new insights into the proteins of structural and nutritional importance in the rumen epithelium, that carry out nutrient transport and metabolism, cell growth and signalling.