Using 4D proteomics, Realis-Doyelle, E., et al. were able to identify 25 neuropeptide precursors involved in the regulation of reproduction in Pacific oysters. By using PEAKS IMS, the researchers were able to take advantage of the 4th-dimension of data generated by the timsTOF Pro.
To study how a change in genetic information, like neuronatin deficiency, has on food intake and body mass, Ciminoet et al. used LC-MS/MS to study the peptides of pancreatic hormones in Nnat deficient and wildtype mice. Learn about this study and how this approach can be used for other studies, like hormone processing, receptor sensitivity, etc.
The link between gut & brain health is a target of interest for Parkinson’s disease (PD) research. In this paper, Gordevicius et al, use multi-molecular methods to study PD pathology from epigenetic changes in DNA & altered transcription, to the resulting modified protein abundance.
Studies about cardiovascular diseases have shown the Elabela/Toddler (ELA) ligand to be a beneficial apelin Rc. agonist. In a recent paper, Nyimanu et al. used LC-MS/MS methods to examine how the different forms of ELA individually impact signaling networks in the human body.
In our food and supplements, we see new functional foods and nutraceuticals with new claims everyday. But do these compounds show any bioactivity to support these claims? Huang et al show Mass spec can be a great tool to study these compound.
In this study, Noori et al show that MS-based MRD monitoring is over 15-fold more sensitive than conventional diagnostic measures. More importantly, MS-based MRD monitoring with PEAKS de novo sequencing can be used with heterogeneous patient populations, and does not have to be adapted when novel t-mAb are approved for use in patients.
A recent study by Karabiyik et al. uncovers key details relating to the signaling pathways of glucose starvation induced autophagy by combining a variety of complimentary analytical techniques, including LC-MS/MS analysis using PEAKS!
Clement et al. have published their method to identify differences in endosomal antigen processing by dendritic cells including wet lab, LC-MS/MS & data analysis. Clear and concise details in this publication, including step-by-step LC-MS/MS and bioinformatic instruction, make this paper an excellent guide for researchers interested in the identification and quantification of antigenic proteins and peptide epitopes.
Li, Yujuan, et al. “Investigation on P-Glycoprotein Function and Its Interacting Proteins under Simulated Microgravity.” Space: Science & Technology, American Association for the Advancement of Science (AAAS), June 2021, pp. 1–13. Crossref, doi:10.34133/2021/9835728.
Mathieu Dupré, Magalie Duchateau, Rebecca Sternke-Hoffmann, Amelie Boquoi, Christian Malosse, Roland Fenk, Rainer Haas, Alexander K. Buell, Martial Rey, and Julia Chamot-Rooke. De Novo Sequencing of Antibody Light Chain Proteoforms from Patients with Multiple Myeloma. Analytical Chemistry 2021 93 (30), 10627-10634. doi:10.1021/acs.analchem.1c01955