Proteogenomic Analysis Unveils the HLA Class I-Presented Immunopeptidome in Melanoma and EGFR-Mutant Lung Adenocarcinoma

One of the hurdles in the development of immunopeptidomic tools is the heterogeneity of the tumour-associated signals resulting in challenges with traditional database search strategies for MS data. Here Qi et al. from NIH are working to remove this hurdle by combining de novo peptide antigen sequencing and custom database strategies.

Transcriptome Profiling of the Pacific Oyster Crassostrea gigas Visceral Ganglia over a Reproduction Cycle Identifies Novel Regulatory Peptides

Using 4D proteomics, Realis-Doyelle, E., et al. were able to identify 25 neuropeptide precursors involved in the regulation of reproduction in Pacific oysters. By using PEAKS IMS, the researchers were able to take advantage of the 4th-dimension of data generated by the timsTOF Pro.

Murine neuronatin deficiency is associated with a hypervariable food intake and bimodal obesity

To study how a change in genetic information, like neuronatin deficiency, has on food intake and body mass, Ciminoet et al. used LC-MS/MS to study the peptides of pancreatic hormones in Nnat deficient and wildtype mice. Learn about this study and how this approach can be used for other studies, like hormone processing, receptor sensitivity, etc.

In vitro metabolism of synthetic Elabela/Toddler (ELA-32) peptide in human plasma and kidney homogenates analyzed with mass spectrometry and validation of endogenous peptide quantification in tissues by ELISA

Studies about cardiovascular diseases have shown the Elabela/Toddler (ELA) ligand to be a beneficial apelin Rc. agonist. In a recent paper, Nyimanu et al. used LC-MS/MS methods to examine how the different forms of ELA individually impact signaling networks in the human body.