In this study, Noori et al show that MS-based MRD monitoring is over 15-fold more sensitive than conventional diagnostic measures. More importantly, MS-based MRD monitoring with PEAKS de novo sequencing can be used with heterogeneous patient populations, and does not have to be adapted when novel t-mAb are approved for use in patients.
Glucose starvation induces autophagy via ULK1-mediated activation of PIKfyve in an AMPK-dependent manner
A recent study by Karabiyik et al. uncovers key details relating to the signaling pathways of glucose starvation induced autophagy by combining a variety of complimentary analytical techniques, including LC-MS/MS analysis using PEAKS!
A protocol for qualitative and quantitative measurement of endosomal processing using hot spot analysis
Clement et al. have published their method to identify differences in endosomal antigen processing by dendritic cells including wet lab, LC-MS/MS & data analysis. Clear and concise details in this publication, including step-by-step LC-MS/MS and bioinformatic instruction, make this paper an excellent guide for researchers interested in the identification and quantification of antigenic proteins and peptide epitopes.
Investigation on P-Glycoprotein Function and Its Interacting Proteins under Simulated Microgravity
Li, Yujuan, et al. “Investigation on P-Glycoprotein Function and Its Interacting Proteins under Simulated Microgravity.” Space: Science & Technology, American Association for the Advancement of Science (AAAS), June 2021, pp. 1–13. Crossref, doi:10.34133/2021/9835728.
De Novo Sequencing of Antibody Light Chain Proteoforms from Patients with Multiple Myeloma
Mathieu Dupré, Magalie Duchateau, Rebecca Sternke-Hoffmann, Amelie Boquoi, Christian Malosse, Roland Fenk, Rainer Haas, Alexander K. Buell, Martial Rey, and Julia Chamot-Rooke. De Novo Sequencing of Antibody Light Chain Proteoforms from Patients with Multiple Myeloma. Analytical Chemistry 2021 93 (30), 10627-10634. doi:10.1021/acs.analchem.1c01955
Synergistic sequence contributions bias glycation outcomes
McEwen, J.M., Fraser, S., Guir, A.L.S. et al. Synergistic sequence contributions bias glycation outcomes. Nat Commun 12, 3316 (2021). doi:10.1038/s41467-021-23625-8
Placental secretome characterization identifies candidates for pregnancy complications
Napso, T., Zhao, X., Lligoña, M.I. et al. Placental secretome characterization identifies candidates for pregnancy complications. Commun Biol 4, 701 (2021). doi:10.1038/s42003-021-02214-x
Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns
Green Daniel R., et al, Mapping the Tooth Enamel Proteome and Amelogenin Phosphorylation Onto Mineralizing Porcine Tooth Crowns, Frontiers in Physiology. V. 10, 2019, 295
The MHC-II peptidome of pancreatic islets identifies key features of autoimmune peptides
Purcell, A.W., Ramarathinam, S.H. & Ternette, N. Mass spectrometry–based identification of MHC-bound peptides for immunopeptidomics. Nat Protoc 14, 1687–1707 (2019)
Mass Spectrometry–based Identification of MHC-bound Peptides for Immunopeptidomics
Purcell, A.W., Ramarathinam, S.H. & Ternette, N. Mass spectrometry–based identification of MHC-bound peptides for immunopeptidomics. Nat Protoc 14, 1687–1707 (2019)