Translation events that were previously thought to result in non-biologically relevant proteins are now being reconsidered. Increasing evidence shows that some non-canonical proteins may play an important role in immunity and disease. This paper examines the unique features of the non-canonical translatome and how they may influence tumor immunosurveillance.
Suprabasin-derived bioactive peptides identified by plasma peptidomics
Isolating and identifying native peptides in serum samples can be very difficult by LC-MS/MS data in plasma studies. Taguchi and colleagues address these challenges and show that this approach to plasma peptidomics can ID unknown low abundance peptides with biological activity.
Proteogenomic Analysis Unveils the HLA Class I-Presented Immunopeptidome in Melanoma and EGFR-Mutant Lung Adenocarcinoma
One of the hurdles in the development of immunopeptidomic tools is the heterogeneity of the tumour-associated signals resulting in challenges with traditional database search strategies for MS data. Here Qi et al. from NIH are working to remove this hurdle by combining de novo peptide antigen sequencing and custom database strategies.
Human Blood and Bird Egg Proteins Identified in Red Paint Covering a 1000-Year-Old Gold Mask from Peru
In a recent study by Pires et al., LC-MS/MS was used to study the protein components of the binding material in a paint sample that remained on a 1000-Year-Old Gold Mask, presumably by members of the Sicán culture of Peru.
RHybridFinder: An R package to process immunopeptidomic data for putative hybrid peptide discovery
Immunopeptidomics is the study of the processed peptides by LC-MS/MS and serves as a tool for the identification of novel antigens. This paper by Saab and others presents a novel statistical computing method called RHybridFinder (RHF).
An Integrated Strategy for Mass Spectrometry-Based Multiomics Analysis of Single Cells
Looking to optimize methods used for single cell proteomics from sample capture, to peptide extraction, through LC-MS/MS & data analysis? Li et al. recently published a workflow for integrated proteome, phosphoproteome, & metabolome analysis from a single cell that addresses many single cell proteomics challenges.
Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in Neonatal Piglet Model
Rosa, Fernanda, et al. “Milk Formula Diet Alters Bacterial and Host Protein Profile in Comparison to Human Milk Diet in...
Extraction and Chemical Characterization of Functional Phenols and Proteins from Coffee (Coffea arabica) By-Products
Prandi, Barbara, et al. “Extraction and Chemical Characterization of Functional Phenols and Proteins from Coffee (Coffea Arabica) By-Products.” Biomolecules, no....
The legumain McPAL1 from Momordica cochinchinensis is a highly stable Asx-specific splicing enzyme
Liew, Heng Tai, et al. “The Legumain McPAL1 from Momordica Cochinchinensis Is a Highly Stable Asx-Specific Splicing Enzyme.” Journal of...
Episymbiotic Saccharibacteria suppresses gingival inflammation and bone loss in mice through host bacterial modulation
Chipashvili, Otari, et al. “Episymbiotic Saccharibacteria Suppresses Gingival Inflammation and Bone Loss in Mice through Host Bacterial Modulation.” Cell Host...