Vasilieva, A. D., et al. “Development of a Diagnostic Approach Based on the Detection of Post-Translation Modifications of Fibrinogen Associated with Oxidative Stress by the Method of High Efficiency Liquid Chromatography.” Russian Journal of Physical Chemistry B 16.1 (2022): 118-122. https://doi.org/10.1134/S1990793122010316

Abstract

In this study, a method is developed for the simplified preparation of samples for analysis by high performance liquid chromatography and tandem mass spectrometry (HPLC–TMS) for further clinical diagnostic use, including the immunoprecipitation of fibrinogen on magnetic microparticles followed by the enzymatic hydrolysis of the protein. Development of an effective technique that would include the isolation of the target protein from the studied plasma sample or any other physiological fluid with the exclusion of a large number of process steps (for example, numerous stages of reprecipitation and various types of chromatography for the isolation of target proteins) and the use of aggressive conditions (for example, for the elution of proteins from carriers), as well as with a decrease in the total labor intensity and time of preparation of samples for analysis, is necessary for use in proteomic studies.