Proteomic analysis of the responses to chelerythrine in the fungal pathogen Ustilaginoidea virens

Wei, Qing-Hui, et al. “Proteomic analysis of the responses to chelerythrine in the fungal pathogen Ustilaginoidea virens.” European Journal of Plant Pathology (2023): 1-14.


Chelerythrine (CHE) is a natural benzophenanthridine alkaloid with broad pharmacological activities. Herein, we investigated the responses to CHE of the fungal pathogen Ustilaginoidea virens using a proteomics approach. At 7.5 × 10–3 mg/mL, the U. virens inhibition rate reached 56.1%, significantly more potent than another two isoquinoline alkaloids and the commercial fungicide validamycin. The disease control effect on plants in the field was > 50% after spraying with 500 × liquid containing 20% CHE, significantly better than that of validamycin. A total of 4734 proteins were identified in CHE-treated U. virens using tandem mass tag (TMT)-based quantitative proteomics, and 823 were differentially expressed proteins (DEPs). Gene Ontology (GO) of DEPs yielded 107 biological process (BP) subcategories, 45 cellular component (CC) subcategories and 43 molecular function (MF) subcategories. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were employed, and seven proteins were selected for subsequent parallel reaction monitoring (PRM) analysis. The results indicate that CHE might lead to apoptosis, but further studies are necessary.