Purification and identification of dual-enzyme hydrolysates obtained from defatted walnut and its antioxidant effects on D-galactose-induced aging mice

Jia, Qi, et al. “Purification and Identification of Dual-Enzyme Hydrolysates Obtained from Defatted Walnut and Its Antioxidant Effects on d-Galactose-Induced Aging Mice.” Journal of Food Measurement and Characterization, Springer Science and Business Media LLC, Oct. 2020. Crossref, doi:10.1007/s11694-020-00702-y.


The purpose of this study was to explore the antioxidant activity in vivo of dual-enzyme hydrolysates obtained from defatted walnut, and to purify and identify it. The walnut protein hydrolysates (WPH) were obtained through two-step enzymatic hydrolysis of alkaline protease and trypsin. The effects of WPH on the oxidative stress-related biochemical indexes of D-galactose-treated mice were investigated. Next, WPH was passed through a Sephadex G-25 gel chromatography, anion exchange chromatography and Sephadex G-15 gel chromatography. Subsequently, peptides in the high antioxidant activity fraction were identified by RP-nano-HPLC–MS/MS. Finally, 14 potential peptides of length 11 AAs to 21 AAs with antioxidant activity were identified from WPH.