Biofilm formation risk assessment for psychrotrophic Pseudomonas in raw milk by MALDI-TOF mass spectrometry

Yan, Minghui, et al. “Biofilm formation risk assessment for psychrotrophic pseudomonas in raw milk by MALDI-TOF mass spectrometry.” LWT (2023): 114508.


Psychrotrophic Pseudomonas is often found as the predominant bacteria causing the spoilage of raw milk during product processing and storage. The spoilage can be aggravated if the strains have biofilm-forming ability. Risk assessment of biofilm formation by psychrotrophic Pseudomonas is highly important to prevent product spoilage at early stage. Herein, we develop an efficient strategy to identify the biofilm-forming risk by psychrotrophic Pseudomonas in raw milk based on MALDI-TOF MS. Molecular mass fingerprinting of whole cells was collected by MALDI-TOF MS, followed by multivariate statistical analysis combined with machine learning to establish a classification model of biofilm-forming ability by psychrotrophs. The results showed that Pseudomonas with different biofilm-forming capabilities can be well discriminated by the MALDI-TOF MS molecular mass fingerprinting. A classification model was established, showing 100% accuracy in biofilm-forming risk identification.